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e 322 human usp2 catalytic domain r d systems  (R&D Systems)


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    R&D Systems e 322 human usp2 catalytic domain r d systems
    E 322 Human Usp2 Catalytic Domain R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e+322+human+usp2+catalytic+domain+r+d+systems/Recombinant+Human+USP2+Catalytic+Domain+Protein%2C+CF/pm41270756-225-286-291
    Average 94 stars, based on 50 article reviews
    e 322 human usp2 catalytic domain r d systems - by Bioz Stars, 2026-09
    94/100 stars

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    Virus:

    Article Title: Deubiquitinases cleave ubiquitin-fused ribosomal proteins and physically counteract their targeting to the UFD pathway.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-HA.11 Epitope Tag Biolegend Cat# 902301; RRID: AB_2565018 Mouse monoclonal anti-alpha-Tubulin Millipore Sigma Cat# CP06; RRID: AB_2617116 Mouse monoclonal anti-BYSTIN Santa Cruz Biotechnology Cat# sc-271722; RRID: AB_10707663 Rabbit anti-EIF6 Cell Signaling Cat# 3833; RRID: AB_2096520 Rabbit polyclonal anti-RPL26 Thermo Fisher Scientific Cat# A300-686A; RRID: AB_530289 Rabbit polyclonal anti-RPS3 Thermo Fisher Scientific Cat# A303-841A; RRID: AB_2620192 Rabbit polyclonal anti-USP7 Thermo Fisher Scientific Cat# A300-033A; RRID: AB_203276 Rabbit polyclonal anti-UCHL3 Thermo Fisher Scientific Cat# A302-948A; RRID: AB_10691115 Mouse monoclonal anti-FLAG Millipore Sigma Cat# F1804; RRID:AB_262044 Rabbit polyclonal anti-HUWE1 Bethyl Cat# A300-486A; RRID:AB_2264590 Rabbit polyclonal anti-TRIP12 Proteintech Cat# 25303-1-AP; RRID:AB_2880020 Rabbit polyclonal anti-UBE2O Bethyl Cat# A301-873A; RRID:AB_1309799 Rabbit monoclonal anti-UBR5 Cell Signaling Cat# 65344; RRID:AB_2799679 Rabbit polyclonal anti-HECTD1 Bethyl Cat# A302-908A; RRID:AB_10664800 Bacterial and virus strains BL21(DE3) New England Biolabs Cat# C2527H Chemicals, peptides, and recombinant proteins FuGENE6 Transfection Reagent Promega Cat# E2691 InSolution MG-132, Proteasome Inhibitor Millipore Sigma Cat# 474791 Ub-VS, Recombinant Human Ubiquitin Vinyl Sulfone R&D Systems Cat# U-202 Biotin-Ahx-Ub(1-75)-Dha-Ub UbiQ Cat# UbiQ-121 10XHis-Ub(1-75)-Dha-RPL40 This study N/A 10XHis-Ub(1-75)-Dha-RPS27A This study N/A Human FAM105B/OTULIN Protein R&D Systems Cat# E-558 Human OTUD7B/Cezanne Protein R&D Systems Cat# E-562 Human OTUD7B/Cezanne Catalytic Domain R&D Systems Cat# E-563 Human USP4 Protein R&D Systems Cat# E-596 Human OTUB1 Protein UBIQUIGENT Cat# 64-0011-050 Human USP11 Protein R&D Systems Cat# E-602 Human UCH-L1 Protein R&D Systems Cat# E-340 Human UCH-L3 Protein R&D Systems Cat# E-325 Human ZRANB1/Trabid Protein R&D Systems Cat# E-560 Human USP7 Protein R&D Systems Cat# E-519 Human USP7 Catalytic Domain R&D Systems Cat# E-518B Human USP15 Protein R&D Systems Cat# E-594 Human USP9x Isoform 2 Protein R&D Systems Cat# E-552 Human USP19 Protein R&D Systems Cat# E-576 Human USP5/Isopeptidase T Protein R&D Systems Cat# E-322 Human USP2 Catalytic Domain R&D Systems Cat# E-504-050 SARS1 PLpro LifeSensors Cat# DB-0602-0025 UBE1 R&D Systems Cat# E-304 UbcH5c R&D Systems Cat# E2-627 HA-Ubiquitin R&D Systems Cat# U-110 (Continued on next page) e1 Molecular Cell 85, 1–11.e1–e7, December 4, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Ub-RP Interactors Proteomics MassIVE Accession number MSV000098468 Uncropped imaging data (gels and blots) This study; Mendeley Data https://doi.org/10.17632/cr5kcb9822.1 Experimental models: Cell lines HEK293T ATCC ATCC Cat# CRL-3216, RRID:CVCL_0063 Oligonucleotides Oligonucleotides (siRNA targeting sequences and primers) Qiagen and This Study Table S2 Recombinant DNA pcDNA3.1_Ub-RPS27A-HA (pSP213) This study N/A pcDNA3.1_RPS27A-HA (pSP216) This study N/A pcDNA3.1_Ub(L73P)-RPS27A-HA (pSP214) This study N/A pcDNA3.1_Ub(GGAA)-RPS27A-HA (pSP215) This study N/A pcDNA3.1_Ub(K0)-RPS27A-HA (pSP217) This study N/A pcDNA3.1_Ub(K0, L73P)-RPS27A-HA (pSP218) This study N/A pcDNA3.1_Ub(K29R, L73P)-RPS27A-HA (pSP259) This study N/A pcDNA3.1_Ub(K48R, L73P)-RPS27A-HA (pSP260) This study N/A pcDNA3.1_Ub(K29,48R, L73P)-RPS27AHA (pSP261) This study N/A pcDNA3.1_Ub(L73P, GGAA)-RPS27A-HA (pSP283) This study N/A pcDNA3.1_Ub(K0, L73P)-RPS27AFLAG (pSP77) This study N/A pcDNA3.1_Ub-RPL40-HA (pSP219) This study N/A pcDNA3.1_RPL40-HA (pSP222) This study N/A pcDNA3.1_Ub(L73P)-RPL40-HA (pSP220) This study N/A pcDNA3.1_Ub(GGAA)-RPL40-HA (pSP221) This study N/A pcDNA3.1_Ub(K0)-RPL40-HA (pSP223) This study N/A pcDNA3.1_Ub(K0, L73P)-RPL40-HA (pSP224) This study N/A pcDNA3.1_Ub(K6+, L73P)-RPL40-HA (pSP249) This study N/A pcDNA3.1_Ub(K11+, L73P)-RPL40-HA (pSP250) This study N/A pcDNA3.1_Ub(K27+, L73P)-RPL40-HA (pSP251) This study N/A pcDNA3.1_Ub(K29+, L73P)-RPL40-HA (pSP252) This study N/A pcDNA3.1_Ub(K33+, L73P)-RPL40-HA (pSP253) This study N/A pcDNA3.1_Ub(K48+, L73P)-RPL40-HA (pSP254) This study N/A pcDNA3.1_Ub(K63+, L73P)-RPL40-HA (pSP255) This study N/A pcDNA3.1_Ub(K29R, L73P)-RPL40-HA (pSP256) This study N/A pcDNA3.1_Ub(K48R, L73P)-RPL40-HA (pSP257) This study N/A (Continued on next page) Molecular Cell 85, 1–11.e1–e7, December 4, 2025 e2

    Recombinant:

    Article Title: Deubiquitinases cleave ubiquitin-fused ribosomal proteins and physically counteract their targeting to the UFD pathway.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-HA.11 Epitope Tag Biolegend Cat# 902301; RRID: AB_2565018 Mouse monoclonal anti-alpha-Tubulin Millipore Sigma Cat# CP06; RRID: AB_2617116 Mouse monoclonal anti-BYSTIN Santa Cruz Biotechnology Cat# sc-271722; RRID: AB_10707663 Rabbit anti-EIF6 Cell Signaling Cat# 3833; RRID: AB_2096520 Rabbit polyclonal anti-RPL26 Thermo Fisher Scientific Cat# A300-686A; RRID: AB_530289 Rabbit polyclonal anti-RPS3 Thermo Fisher Scientific Cat# A303-841A; RRID: AB_2620192 Rabbit polyclonal anti-USP7 Thermo Fisher Scientific Cat# A300-033A; RRID: AB_203276 Rabbit polyclonal anti-UCHL3 Thermo Fisher Scientific Cat# A302-948A; RRID: AB_10691115 Mouse monoclonal anti-FLAG Millipore Sigma Cat# F1804; RRID:AB_262044 Rabbit polyclonal anti-HUWE1 Bethyl Cat# A300-486A; RRID:AB_2264590 Rabbit polyclonal anti-TRIP12 Proteintech Cat# 25303-1-AP; RRID:AB_2880020 Rabbit polyclonal anti-UBE2O Bethyl Cat# A301-873A; RRID:AB_1309799 Rabbit monoclonal anti-UBR5 Cell Signaling Cat# 65344; RRID:AB_2799679 Rabbit polyclonal anti-HECTD1 Bethyl Cat# A302-908A; RRID:AB_10664800 Bacterial and virus strains BL21(DE3) New England Biolabs Cat# C2527H Chemicals, peptides, and recombinant proteins FuGENE6 Transfection Reagent Promega Cat# E2691 InSolution MG-132, Proteasome Inhibitor Millipore Sigma Cat# 474791 Ub-VS, Recombinant Human Ubiquitin Vinyl Sulfone R&D Systems Cat# U-202 Biotin-Ahx-Ub(1-75)-Dha-Ub UbiQ Cat# UbiQ-121 10XHis-Ub(1-75)-Dha-RPL40 This study N/A 10XHis-Ub(1-75)-Dha-RPS27A This study N/A Human FAM105B/OTULIN Protein R&D Systems Cat# E-558 Human OTUD7B/Cezanne Protein R&D Systems Cat# E-562 Human OTUD7B/Cezanne Catalytic Domain R&D Systems Cat# E-563 Human USP4 Protein R&D Systems Cat# E-596 Human OTUB1 Protein UBIQUIGENT Cat# 64-0011-050 Human USP11 Protein R&D Systems Cat# E-602 Human UCH-L1 Protein R&D Systems Cat# E-340 Human UCH-L3 Protein R&D Systems Cat# E-325 Human ZRANB1/Trabid Protein R&D Systems Cat# E-560 Human USP7 Protein R&D Systems Cat# E-519 Human USP7 Catalytic Domain R&D Systems Cat# E-518B Human USP15 Protein R&D Systems Cat# E-594 Human USP9x Isoform 2 Protein R&D Systems Cat# E-552 Human USP19 Protein R&D Systems Cat# E-576 Human USP5/Isopeptidase T Protein R&D Systems Cat# E-322 Human USP2 Catalytic Domain R&D Systems Cat# E-504-050 SARS1 PLpro LifeSensors Cat# DB-0602-0025 UBE1 R&D Systems Cat# E-304 UbcH5c R&D Systems Cat# E2-627 HA-Ubiquitin R&D Systems Cat# U-110 (Continued on next page) e1 Molecular Cell 85, 1–11.e1–e7, December 4, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Ub-RP Interactors Proteomics MassIVE Accession number MSV000098468 Uncropped imaging data (gels and blots) This study; Mendeley Data https://doi.org/10.17632/cr5kcb9822.1 Experimental models: Cell lines HEK293T ATCC ATCC Cat# CRL-3216, RRID:CVCL_0063 Oligonucleotides Oligonucleotides (siRNA targeting sequences and primers) Qiagen and This Study Table S2 Recombinant DNA pcDNA3.1_Ub-RPS27A-HA (pSP213) This study N/A pcDNA3.1_RPS27A-HA (pSP216) This study N/A pcDNA3.1_Ub(L73P)-RPS27A-HA (pSP214) This study N/A pcDNA3.1_Ub(GGAA)-RPS27A-HA (pSP215) This study N/A pcDNA3.1_Ub(K0)-RPS27A-HA (pSP217) This study N/A pcDNA3.1_Ub(K0, L73P)-RPS27A-HA (pSP218) This study N/A pcDNA3.1_Ub(K29R, L73P)-RPS27A-HA (pSP259) This study N/A pcDNA3.1_Ub(K48R, L73P)-RPS27A-HA (pSP260) This study N/A pcDNA3.1_Ub(K29,48R, L73P)-RPS27AHA (pSP261) This study N/A pcDNA3.1_Ub(L73P, GGAA)-RPS27A-HA (pSP283) This study N/A pcDNA3.1_Ub(K0, L73P)-RPS27AFLAG (pSP77) This study N/A pcDNA3.1_Ub-RPL40-HA (pSP219) This study N/A pcDNA3.1_RPL40-HA (pSP222) This study N/A pcDNA3.1_Ub(L73P)-RPL40-HA (pSP220) This study N/A pcDNA3.1_Ub(GGAA)-RPL40-HA (pSP221) This study N/A pcDNA3.1_Ub(K0)-RPL40-HA (pSP223) This study N/A pcDNA3.1_Ub(K0, L73P)-RPL40-HA (pSP224) This study N/A pcDNA3.1_Ub(K6+, L73P)-RPL40-HA (pSP249) This study N/A pcDNA3.1_Ub(K11+, L73P)-RPL40-HA (pSP250) This study N/A pcDNA3.1_Ub(K27+, L73P)-RPL40-HA (pSP251) This study N/A pcDNA3.1_Ub(K29+, L73P)-RPL40-HA (pSP252) This study N/A pcDNA3.1_Ub(K33+, L73P)-RPL40-HA (pSP253) This study N/A pcDNA3.1_Ub(K48+, L73P)-RPL40-HA (pSP254) This study N/A pcDNA3.1_Ub(K63+, L73P)-RPL40-HA (pSP255) This study N/A pcDNA3.1_Ub(K29R, L73P)-RPL40-HA (pSP256) This study N/A pcDNA3.1_Ub(K48R, L73P)-RPL40-HA (pSP257) This study N/A (Continued on next page) Molecular Cell 85, 1–11.e1–e7, December 4, 2025 e2

    Transfection:

    Article Title: Deubiquitinases cleave ubiquitin-fused ribosomal proteins and physically counteract their targeting to the UFD pathway.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-HA.11 Epitope Tag Biolegend Cat# 902301; RRID: AB_2565018 Mouse monoclonal anti-alpha-Tubulin Millipore Sigma Cat# CP06; RRID: AB_2617116 Mouse monoclonal anti-BYSTIN Santa Cruz Biotechnology Cat# sc-271722; RRID: AB_10707663 Rabbit anti-EIF6 Cell Signaling Cat# 3833; RRID: AB_2096520 Rabbit polyclonal anti-RPL26 Thermo Fisher Scientific Cat# A300-686A; RRID: AB_530289 Rabbit polyclonal anti-RPS3 Thermo Fisher Scientific Cat# A303-841A; RRID: AB_2620192 Rabbit polyclonal anti-USP7 Thermo Fisher Scientific Cat# A300-033A; RRID: AB_203276 Rabbit polyclonal anti-UCHL3 Thermo Fisher Scientific Cat# A302-948A; RRID: AB_10691115 Mouse monoclonal anti-FLAG Millipore Sigma Cat# F1804; RRID:AB_262044 Rabbit polyclonal anti-HUWE1 Bethyl Cat# A300-486A; RRID:AB_2264590 Rabbit polyclonal anti-TRIP12 Proteintech Cat# 25303-1-AP; RRID:AB_2880020 Rabbit polyclonal anti-UBE2O Bethyl Cat# A301-873A; RRID:AB_1309799 Rabbit monoclonal anti-UBR5 Cell Signaling Cat# 65344; RRID:AB_2799679 Rabbit polyclonal anti-HECTD1 Bethyl Cat# A302-908A; RRID:AB_10664800 Bacterial and virus strains BL21(DE3) New England Biolabs Cat# C2527H Chemicals, peptides, and recombinant proteins FuGENE6 Transfection Reagent Promega Cat# E2691 InSolution MG-132, Proteasome Inhibitor Millipore Sigma Cat# 474791 Ub-VS, Recombinant Human Ubiquitin Vinyl Sulfone R&D Systems Cat# U-202 Biotin-Ahx-Ub(1-75)-Dha-Ub UbiQ Cat# UbiQ-121 10XHis-Ub(1-75)-Dha-RPL40 This study N/A 10XHis-Ub(1-75)-Dha-RPS27A This study N/A Human FAM105B/OTULIN Protein R&D Systems Cat# E-558 Human OTUD7B/Cezanne Protein R&D Systems Cat# E-562 Human OTUD7B/Cezanne Catalytic Domain R&D Systems Cat# E-563 Human USP4 Protein R&D Systems Cat# E-596 Human OTUB1 Protein UBIQUIGENT Cat# 64-0011-050 Human USP11 Protein R&D Systems Cat# E-602 Human UCH-L1 Protein R&D Systems Cat# E-340 Human UCH-L3 Protein R&D Systems Cat# E-325 Human ZRANB1/Trabid Protein R&D Systems Cat# E-560 Human USP7 Protein R&D Systems Cat# E-519 Human USP7 Catalytic Domain R&D Systems Cat# E-518B Human USP15 Protein R&D Systems Cat# E-594 Human USP9x Isoform 2 Protein R&D Systems Cat# E-552 Human USP19 Protein R&D Systems Cat# E-576 Human USP5/Isopeptidase T Protein R&D Systems Cat# E-322 Human USP2 Catalytic Domain R&D Systems Cat# E-504-050 SARS1 PLpro LifeSensors Cat# DB-0602-0025 UBE1 R&D Systems Cat# E-304 UbcH5c R&D Systems Cat# E2-627 HA-Ubiquitin R&D Systems Cat# U-110 (Continued on next page) e1 Molecular Cell 85, 1–11.e1–e7, December 4, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Ub-RP Interactors Proteomics MassIVE Accession number MSV000098468 Uncropped imaging data (gels and blots) This study; Mendeley Data https://doi.org/10.17632/cr5kcb9822.1 Experimental models: Cell lines HEK293T ATCC ATCC Cat# CRL-3216, RRID:CVCL_0063 Oligonucleotides Oligonucleotides (siRNA targeting sequences and primers) Qiagen and This Study Table S2 Recombinant DNA pcDNA3.1_Ub-RPS27A-HA (pSP213) This study N/A pcDNA3.1_RPS27A-HA (pSP216) This study N/A pcDNA3.1_Ub(L73P)-RPS27A-HA (pSP214) This study N/A pcDNA3.1_Ub(GGAA)-RPS27A-HA (pSP215) This study N/A pcDNA3.1_Ub(K0)-RPS27A-HA (pSP217) This study N/A pcDNA3.1_Ub(K0, L73P)-RPS27A-HA (pSP218) This study N/A pcDNA3.1_Ub(K29R, L73P)-RPS27A-HA (pSP259) This study N/A pcDNA3.1_Ub(K48R, L73P)-RPS27A-HA (pSP260) This study N/A pcDNA3.1_Ub(K29,48R, L73P)-RPS27AHA (pSP261) This study N/A pcDNA3.1_Ub(L73P, GGAA)-RPS27A-HA (pSP283) This study N/A pcDNA3.1_Ub(K0, L73P)-RPS27AFLAG (pSP77) This study N/A pcDNA3.1_Ub-RPL40-HA (pSP219) This study N/A pcDNA3.1_RPL40-HA (pSP222) This study N/A pcDNA3.1_Ub(L73P)-RPL40-HA (pSP220) This study N/A pcDNA3.1_Ub(GGAA)-RPL40-HA (pSP221) This study N/A pcDNA3.1_Ub(K0)-RPL40-HA (pSP223) This study N/A pcDNA3.1_Ub(K0, L73P)-RPL40-HA (pSP224) This study N/A pcDNA3.1_Ub(K6+, L73P)-RPL40-HA (pSP249) This study N/A pcDNA3.1_Ub(K11+, L73P)-RPL40-HA (pSP250) This study N/A pcDNA3.1_Ub(K27+, L73P)-RPL40-HA (pSP251) This study N/A pcDNA3.1_Ub(K29+, L73P)-RPL40-HA (pSP252) This study N/A pcDNA3.1_Ub(K33+, L73P)-RPL40-HA (pSP253) This study N/A pcDNA3.1_Ub(K48+, L73P)-RPL40-HA (pSP254) This study N/A pcDNA3.1_Ub(K63+, L73P)-RPL40-HA (pSP255) This study N/A pcDNA3.1_Ub(K29R, L73P)-RPL40-HA (pSP256) This study N/A pcDNA3.1_Ub(K48R, L73P)-RPL40-HA (pSP257) This study N/A (Continued on next page) Molecular Cell 85, 1–11.e1–e7, December 4, 2025 e2

    Ubiquitin Proteomics:

    Article Title: Deubiquitinases cleave ubiquitin-fused ribosomal proteins and physically counteract their targeting to the UFD pathway.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-HA.11 Epitope Tag Biolegend Cat# 902301; RRID: AB_2565018 Mouse monoclonal anti-alpha-Tubulin Millipore Sigma Cat# CP06; RRID: AB_2617116 Mouse monoclonal anti-BYSTIN Santa Cruz Biotechnology Cat# sc-271722; RRID: AB_10707663 Rabbit anti-EIF6 Cell Signaling Cat# 3833; RRID: AB_2096520 Rabbit polyclonal anti-RPL26 Thermo Fisher Scientific Cat# A300-686A; RRID: AB_530289 Rabbit polyclonal anti-RPS3 Thermo Fisher Scientific Cat# A303-841A; RRID: AB_2620192 Rabbit polyclonal anti-USP7 Thermo Fisher Scientific Cat# A300-033A; RRID: AB_203276 Rabbit polyclonal anti-UCHL3 Thermo Fisher Scientific Cat# A302-948A; RRID: AB_10691115 Mouse monoclonal anti-FLAG Millipore Sigma Cat# F1804; RRID:AB_262044 Rabbit polyclonal anti-HUWE1 Bethyl Cat# A300-486A; RRID:AB_2264590 Rabbit polyclonal anti-TRIP12 Proteintech Cat# 25303-1-AP; RRID:AB_2880020 Rabbit polyclonal anti-UBE2O Bethyl Cat# A301-873A; RRID:AB_1309799 Rabbit monoclonal anti-UBR5 Cell Signaling Cat# 65344; RRID:AB_2799679 Rabbit polyclonal anti-HECTD1 Bethyl Cat# A302-908A; RRID:AB_10664800 Bacterial and virus strains BL21(DE3) New England Biolabs Cat# C2527H Chemicals, peptides, and recombinant proteins FuGENE6 Transfection Reagent Promega Cat# E2691 InSolution MG-132, Proteasome Inhibitor Millipore Sigma Cat# 474791 Ub-VS, Recombinant Human Ubiquitin Vinyl Sulfone R&D Systems Cat# U-202 Biotin-Ahx-Ub(1-75)-Dha-Ub UbiQ Cat# UbiQ-121 10XHis-Ub(1-75)-Dha-RPL40 This study N/A 10XHis-Ub(1-75)-Dha-RPS27A This study N/A Human FAM105B/OTULIN Protein R&D Systems Cat# E-558 Human OTUD7B/Cezanne Protein R&D Systems Cat# E-562 Human OTUD7B/Cezanne Catalytic Domain R&D Systems Cat# E-563 Human USP4 Protein R&D Systems Cat# E-596 Human OTUB1 Protein UBIQUIGENT Cat# 64-0011-050 Human USP11 Protein R&D Systems Cat# E-602 Human UCH-L1 Protein R&D Systems Cat# E-340 Human UCH-L3 Protein R&D Systems Cat# E-325 Human ZRANB1/Trabid Protein R&D Systems Cat# E-560 Human USP7 Protein R&D Systems Cat# E-519 Human USP7 Catalytic Domain R&D Systems Cat# E-518B Human USP15 Protein R&D Systems Cat# E-594 Human USP9x Isoform 2 Protein R&D Systems Cat# E-552 Human USP19 Protein R&D Systems Cat# E-576 Human USP5/Isopeptidase T Protein R&D Systems Cat# E-322 Human USP2 Catalytic Domain R&D Systems Cat# E-504-050 SARS1 PLpro LifeSensors Cat# DB-0602-0025 UBE1 R&D Systems Cat# E-304 UbcH5c R&D Systems Cat# E2-627 HA-Ubiquitin R&D Systems Cat# U-110 (Continued on next page) e1 Molecular Cell 85, 1–11.e1–e7, December 4, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Ub-RP Interactors Proteomics MassIVE Accession number MSV000098468 Uncropped imaging data (gels and blots) This study; Mendeley Data https://doi.org/10.17632/cr5kcb9822.1 Experimental models: Cell lines HEK293T ATCC ATCC Cat# CRL-3216, RRID:CVCL_0063 Oligonucleotides Oligonucleotides (siRNA targeting sequences and primers) Qiagen and This Study Table S2 Recombinant DNA pcDNA3.1_Ub-RPS27A-HA (pSP213) This study N/A pcDNA3.1_RPS27A-HA (pSP216) This study N/A pcDNA3.1_Ub(L73P)-RPS27A-HA (pSP214) This study N/A pcDNA3.1_Ub(GGAA)-RPS27A-HA (pSP215) This study N/A pcDNA3.1_Ub(K0)-RPS27A-HA (pSP217) This study N/A pcDNA3.1_Ub(K0, L73P)-RPS27A-HA (pSP218) This study N/A pcDNA3.1_Ub(K29R, L73P)-RPS27A-HA (pSP259) This study N/A pcDNA3.1_Ub(K48R, L73P)-RPS27A-HA (pSP260) This study N/A pcDNA3.1_Ub(K29,48R, L73P)-RPS27AHA (pSP261) This study N/A pcDNA3.1_Ub(L73P, GGAA)-RPS27A-HA (pSP283) This study N/A pcDNA3.1_Ub(K0, L73P)-RPS27AFLAG (pSP77) This study N/A pcDNA3.1_Ub-RPL40-HA (pSP219) This study N/A pcDNA3.1_RPL40-HA (pSP222) This study N/A pcDNA3.1_Ub(L73P)-RPL40-HA (pSP220) This study N/A pcDNA3.1_Ub(GGAA)-RPL40-HA (pSP221) This study N/A pcDNA3.1_Ub(K0)-RPL40-HA (pSP223) This study N/A pcDNA3.1_Ub(K0, L73P)-RPL40-HA (pSP224) This study N/A pcDNA3.1_Ub(K6+, L73P)-RPL40-HA (pSP249) This study N/A pcDNA3.1_Ub(K11+, L73P)-RPL40-HA (pSP250) This study N/A pcDNA3.1_Ub(K27+, L73P)-RPL40-HA (pSP251) This study N/A pcDNA3.1_Ub(K29+, L73P)-RPL40-HA (pSP252) This study N/A pcDNA3.1_Ub(K33+, L73P)-RPL40-HA (pSP253) This study N/A pcDNA3.1_Ub(K48+, L73P)-RPL40-HA (pSP254) This study N/A pcDNA3.1_Ub(K63+, L73P)-RPL40-HA (pSP255) This study N/A pcDNA3.1_Ub(K29R, L73P)-RPL40-HA (pSP256) This study N/A pcDNA3.1_Ub(K48R, L73P)-RPL40-HA (pSP257) This study N/A (Continued on next page) Molecular Cell 85, 1–11.e1–e7, December 4, 2025 e2



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    https://www.bioz.com/product/e+322+human+usp2+catalytic+domain+r+d+systems/Recombinant+Human+USP2+Catalytic+Domain+Protein%2C+CF/pm41270756-225-286-291
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